Effects of trace element deficiency on drug-metabolizing enzymes in rats
نویسندگان
چکیده
We investigated the effects of trace element deficiency on drug-metabolizing enzymes in rats. Weanling rats were fed a selenium-deficient diet (Torula yeast) or a normal diet (0.5μg selenium) until 20 weeks. After six weeks of the selenium-deficient diet, an increase in aniline hydroxylase activity was observed in the selenium-deficient rats, but the activity of aminopyrine N-demethylase was reduced. In three-week-old Wistar rats randomly assigned to three groups: a zinc-adequate ad libitum group, a zinc-adequate pair-fed group, and a zinc-deficient group, zinc deficiency resulted in decreased aminopyrine N-demethylase, aniline hydroxylase and p-nitroanisole o-demethylase activities. Moreover, Western immunoblot analysis using CYP 2C11 antibody revealed an inhibition of CYP 2C11 protein synthesis in zinc-deficient rats at five weeks. The results of this study suggest that drug-metabolizing enzymes are affected by selenium and zinc deficiency in rats.
منابع مشابه
The effects of fenvalerate on hepatic and cerebral xenobiotic metabolizing enzymes in selenium and/or iodine deficient rats
Objective(s): Particularly in developing countries, selenium and/or iodine deficiencies are encountered and use of pesticides in agriculture are not well-controlled. Fenvalerate is a pyrethroid insectide used in agriculture and has applications against a wide range of pests. This study was designed to evaluate the effects of fenvalerate on hepatic and cerebral xenobiotic metabolizing enzyme act...
متن کاملEffects of salidroside on rat CYP enzymes by a cocktail of probe drugs
Objective(s): In this study, we aimed to evaluate the effect of salidroside on the activities of the different drug-metabolizing enzymes CYP1A2, CYP2B6, CYP2C9, CYP2D6 and CYP3A4 in rats, in which a specific probe drug was used for each enzyme. Materials and Methods: After pretreatment with salidroside, five probe drugs were simultaneously administered to rats by gavage. The given dose was 2.0 ...
متن کاملAlteration of drug metabolizing enzymes in sulphite oxidase deficiency
The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency....
متن کاملEffects of Hyperthyroidism on the Serum Levels of Vanadium and Manganese trace Elements in Rats
Introduction: Trace elements are important for the normal function of thyroid and prevention of its diseases. Normal activities of thyroid including the synthesis of thyroid hormones and regulation of metabolism are dependent on the presence of trace elements. On the other hand, thyroid hormones may also affect the trace element levels. In this study, we investigated the effects of induced h...
متن کاملPolychlorinated dibenzofurans--potent inducers of rat hepatic drug-metabolizing enzymes.
The effects of polychlorinated dibenzofurans (PCDFs), trace toxic contaminants of commercial polychlorinated biphenyl preparations (PCBs), on the induction of hepatic drug-metabolizing enzymes were studied in the rat. PCDFs were about a thousand times more potent than PCBs (Kanechlor-500) as inducers of cytochrome P-450. Rats given 10 microgram/kg of PCDFs intraperitoneally for 3 days showed si...
متن کامل